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Promega mmlv-rt buffer (5× promega)
Mmlv Rt Buffer (5× Promega), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mmlv-rt+buffer+(5%C3%97+promega)/m+mlvrt+h+++enzyme/us08114670-307-15-18
Average 90 stars, based on 1 article reviews
mmlv-rt buffer (5× promega) - by Bioz Stars, 2026-10
90/100 stars

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Article Title: A Novel RNA Virus, Macrobrachium rosenbergii Golda Virus (MrGV), Linked to Mass Mortalities of the Larval Giant Freshwater Prawn in Bangladesh
Article Snippet: First strand synthesis was performed in 25 μL reaction volumes using 5 μL of MMLV-RT 5× buffer (Promega, Madison, WI, USA), 1.25 μL of dNTPs (10 mM) (Promega), 0.625 μL of recombinant RNasin (Promega), 1 μL of random hexamer primers (Promega) and 1 μg of RNA in 16.125 μL.

Article Title: HMGA2 regulates GPX4 expression and ferroptosis in prostate cancer cells.
Article Snippet: Prostate cancer (PCa) remains a significant global health burden and an increase in oxidative stress is associated with cancer progression.. High Mobility Group A2 (HMGA2), a chromatin architectural protein, increases oxidative stress and promotes sensitivity to ferroptosis inducers, however, the mechanism is unknown.. We investigated the role of HMGA2 in GPX4 regulation and the impact on cellular responses to oxidative stress and ferroptosis sensitivity.

Article Title: TGF-β2, catalase activity, H 2 O 2 output and metastatic potential of diverse types of tumour.
Article Snippet: Afterwards, 8μl of reaction solution (0.5μL random hexamer, 4μL 5x RT buffer, 1.5μL 10mM dNTP, 1μL 200U/μLRT-MMLV (Promega) and 1μL 40U/μLRNase inhibitor (Promega) was added to get a final reaction volume of 20μL and incubated at 37°C for 2 h. The resultant cDNA was stored at -20°C.

Article Title: TGF-β2, catalase activity, H 2 O 2 output and metastatic potential of diverse types of tumour
Article Snippet: Afterwards, 8μl of reaction solution (0.5μL random hexamer, 4μL 5x RT buffer, 1.5μL 10mM dNTP, 1μL 200U/μLRT-MMLV (Promega) and 1μL 40U/μLRNase inhibitor (Promega) was added to get a final reaction volume of 20μL and incubated at 37°C for 2 h. The resultant cDNA was stored at −20°C.

Article Title: A novel RNA virus, Macrobrachium rosenbergii Golda virus (MrGV), linked to mass mortalities of the larval giant freshwater prawn in Bangladesh
Article Snippet: First strand synthesis was performed in 25 μl reaction volumes using 5 μl of MMLV-RT 5X buffer (Promega, Wisconsin, UK), 1.25 μl of dNTPs (10mM) (Promega), 0.625 μl of recombinant RNasin (Promega), 1 μl of random hexamer primers (Promega) and 1 μg of RNA in 16.125 μl.

Article Title: Cell penetrating peptides to dissect host-pathogen protein-protein interactions in Theileria -transformed leukocytes
Article Snippet: Afterwards, 8 μl of reaction solution (0.5 μL random hexamer, 4 μL 5× RT buffer, 1.5 μL 10 mM dNTP, 1 μL 200 U/μLRT-MMLV (Promega) and 1 μL 40 U/μLR-Nase inhibitor (Promega) was added to get a final reaction volume of 20 μL and incubated at 37 °C for 2 h. The resultant RNA was stored at −20 °C.

Reverse Transcription:

Article Title: Transcriptional and functional characterization of neonatal circulating Innate Lymphoid Cells
Article Snippet: .. Then, a second master mix containing 5× MMLV RT buffer, MMLV reverse transcriptase, RNAsin (all from Promega, Fitchburg, Wisconsin), and dNTPs (10 nmol/μL, Peqlab) was added and the reverse transcription reaction was run for 100 minutes at 42°C and 15 minutes at 72°C. .. Gene expression analyses was done using Taqman Universal Master Mix II (with UNG, Applied Biosystems, Waltham, Massachusetts), Taqman probes for GAPDH (Hs02786624_g1), ID3 (Hs00171409‐m1), and ID2 (Hs04187239‐m1) were purchased from Thermo Fischer Scientific.

Article Title: Bacteria are important dimethylsulfoniopropionate producers in marine aphotic and high-pressure environments.
Article Snippet: For reverse-transcription qPCR, 9 μl of purified RNA was mixed with 1 μl random hexamer primers (Invitrogen). .. The reaction system was incubated at 70 °C for 5 min and cooled on ice, before mixing with 1 μl dNTPs (10 mM), 4 μl of MMLV RT 5× buffer (Promega), 0.8 μl of M-MLV reverse transcriptase (Promega), 0.4 μl of RNase inhibitor (40 U μl−1, Roche), and 3.8 μl of Diethyl Pyrocarbonate (DEPC) water. ..

Incubation:

Article Title: Bacteria are important dimethylsulfoniopropionate producers in marine aphotic and high-pressure environments.
Article Snippet: For reverse-transcription qPCR, 9 μl of purified RNA was mixed with 1 μl random hexamer primers (Invitrogen). .. The reaction system was incubated at 70 °C for 5 min and cooled on ice, before mixing with 1 μl dNTPs (10 mM), 4 μl of MMLV RT 5× buffer (Promega), 0.8 μl of M-MLV reverse transcriptase (Promega), 0.4 μl of RNase inhibitor (40 U μl−1, Roche), and 3.8 μl of Diethyl Pyrocarbonate (DEPC) water. ..



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Promega mmlv-rt buffer (5× promega)
Mmlv Rt Buffer (5× Promega), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mmlv-rt+buffer+(5%C3%97+promega)/m+mlvrt+h+++enzyme/us08114670-307-15-18
Average 90 stars, based on 1 article reviews
mmlv-rt buffer (5× promega) - by Bioz Stars, 2026-10
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Promega pcr reagents (mmlv-rt, rnasin, 5 mmlv buffer, hexamer, bovine serum albumin, and deoxynucleotide triphosphate; promega)
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